Create Control File

A helper function to draft a description of your single stained control files such that AutoSpectral can understand and process them correctly. Given a set of single stained control fcs files, create.control.file will produce a csv file listing the matching peak detector channels for your fluorophores (if known). If your files contain bead or cell tags in the filename, it will assign your controls as cells or beads. You will need to fill in any "No Match" results manually. You will need to set universal negatives manually. You will need to add marker names manually.

Usage

create.control.file(
  control.dir,
  asp,
  fill.gate.name = TRUE,
  filename = "fcs_control_file"
)

Arguments

control.dir

file path to the single stained control fcs files

asp

The AutoSpectral parameter list. Generate using get.autospectral.param

fill.gate.name

Logical, default is TRUE. Will attempt to automatically assign gate names for the gate.name column if TRUE.

filename

Character string defining the output filename. Default is "fcs_control_file", to which .csv will be appended.

Value

No returns. Outputs a csv file called fcs_control_file.csv