Per-Detector Spectral Distance Between Particle Types

For each fluorophore present in cell.variants, computes the raw per-detector difference (cell reference spectrum minus bead reference spectrum) between two particle types' extracted reference spectra.

cell.variants and bead.variants are expected to come from independent get.spectral.variants() calls (e.g. one per particle-type folder) and are not guaranteed to share the same detector set or column order, so the comparison is aligned explicitly by detector name rather than position. A warning is issued if the two particle types were extracted with different detector sets.

Usage

bead.cell.dist(cell.variants, bead.variants)

Arguments

cell.variants

Named list of spectral variant matrices (one per fluorophore), as returned in the variants element of get.spectral.variants(), for the reference particle type (typically Cells). Row 1 of each matrix is treated as the reference spectrum.

bead.variants

Named list of spectral variant matrices in the same format as cell.variants, for the particle type being compared against the reference (e.g. a bead type).

Value

A numeric matrix of per-detector differences (cell minus bead), one row per fluorophore common to both lists, columns are the shared detector channels.