unmix.ols

Performs spectral unmixing using ordinary least squares

unmix.ols(raw.data, spectra, weights = NULL)

Arguments

raw.data

Expression data from raw fcs files. Cells in rows and detectors in columns. Columns should be fluorescent data only and must match the columns in spectra.

spectra

Spectral signatures of fluorophores, normalized between 0 and 1, with fluorophores in rows and detectors in columns.

weights

Dummy argument to allow dynamic switching between OLS and WLS. Default is NULL. Values passed to weights will be ignored.

Value

Unmixed data with cells in rows and fluorophores in columns.