This function fits a spline to autofluorescence data, removing extreme events
and defining bounds equally far from zero. It uses robust linear modeling
and identifies events within a specified number of standard deviations from
the spline.
Usage
fit.af.spline(af.cells, non.af.cells, asp)
Arguments
- af.cells
A matrix containing the autofluorescence data.
- non.af.cells
A matrix containing the low autofluorescence data.
- asp
The AutoSpectral parameter list.
Prepare using get.autospectral.param
Value
The boundary of the events within the specified number of standard
deviations from the spline.