Fit Spline to Autofluorescence Data

This function fits a spline to autofluorescence data, removing extreme events and defining bounds equally far from zero. It uses robust linear modeling and identifies events within a specified number of standard deviations from the spline.

fit.af.spline(af.cells, non.af.cells, asp)

Arguments

af.cells

A matrix containing the autofluorescence data.

non.af.cells

A matrix containing the low autofluorescence data.

asp

The AutoSpectral parameter list. Prepare using get.autospectral.param

Value

The boundary of the events within the specified number of standard deviations from the spline.