Do Gate

Performs gating on scatter parameters and returns a vector with the indexes of events inside the initial gate.

The gating proceeds in three steps:

• Defines bounds by data trimming

• Defines a region around the target maximum found within the bounds

• Defines a gate around the target maximum, only within that region

The method uses numerical search of maxima over estimated densities and Voronoi tessellations to improve density estimation around maxima.

do.gate(
  gate.data,
  viability.gate,
  large.gate,
  samp,
  scatter.and.channel.label,
  control.type,
  asp
)

Arguments

gate.data

A data frame containing the gate data.

viability.gate

A logical vector indicating the viability gate.

large.gate

A logical vector indicating the large gate.

samp

A sample identifier.

scatter.and.channel.label

A label for scatter and channel.

control.type

The type of control used, either "beads" or "cells".

asp

The AutoSpectral parameter list, prepared using get.autospectral.param.

Value

A vector with the indexes of events inside the initial gate.