This function returns a vector with the indexes of events inside the initial gate on autofluorescence parameters. It proceeds through several steps to define the gate boundaries and identify density maxima using numerical search and Voronoi tessellations.
do.gate.af(gate.data, samp, asp, intermediate.figures = FALSE)A data frame containing the gate data.
A sample identifier.
The AutoSpectral parameter list.
Prepare using get.autospectral.param
Logical, if TRUE returns additional figures to
show the inner workings of the cleaning, including definition of low-AF cell
gates on the PCA-unmixed unstained and spectral ribbon plots of the AF
exclusion from the unstained.
A vector with the indexes of events inside the initial gate.