Faster solver for per-cell optimization workflow. Performs spectral unmixing using weighted least squares.
Arguments
- raw.data
Expression data from raw fcs files. A single row of cells with detectors in columns. Columns should be fluorescent data only and must match the columns in spectra.
- spectra
Spectral signatures of fluorophores, normalized between 0 and 1, with fluorophores in rows and detectors in columns.
- weights
The weighting values for the detectors. No checks are performed in this function;
unmix.wlsshould be used for most cases.