Launch the Thresholding Shiny App

A simple wrapper function to launch the manual threshold selection app.

Usage

launch.threshold.app(
  flowcode.combo.file = NULL,
  flow.control = NULL,
  spectra = NULL
)

Arguments

flowcode.combo.file

Optional, default is NULL. Provide this when you wish to establish new thresholds using the app and will be working with an FCS file rather than loading the RDS object. When given: File name and path to the CSV file containing the information describing your FlowCode library. Describes the valid combinations of FlowCodes. Structure: One row per combination. Columns are Id, Procode.tag1, Procode.tag2 and Procode.tag3, describing the name (e.g., CRISPR target), and three epitopes for the combination, respectively.

flow.control

Optional, default is NULL. Provide this when you wish to establish new thresholds using the app and will be working with an FCS file rather than loading the RDS object. A list containing flow cytometry control parameters.

spectra

Optional, default is NULL. Provide this when you wish to establish new thresholds using the app and will be working with an FCS file rather than loading the RDS object.Spectral signatures of fluorophores, normalized between 0 and 1, with fluorophores in rows and detectors in columns.